Piezoelectric Sensors by Unknown

Author:Unknown
Language: eng
Format: epub
ISBN: 9783031537851
Publisher: Springer International Publishing

Ye et al. later applied similar concepts to synthesize surface-imprinted polymer beads for bacterial recognition [128], namely Pickering emulsions relying on bacteria cells as the stabilizer. First described in 1907 [129], Pickering emulsions represent the process of forming an emulsion between water and oil phases using solid particles instead of surfactants as the stabilizing agents [130]. Ye and coworkers relied on a first self-assembly step of negatively charged bacteria (i.e., Escherichia coli and Micrococcus luteus) to a positively charged vinyl-containing pre-polymer (i.e., N-acrylchitosan). This bacteria–pre-polymer assembly served as the particle stabilizer for the Pickering emulsion in water. The oil phase consisted of crosslinking, hydrophobic monomers and a radical initiator. FRP was then used to form polymer beads with the covalently attached pre-polymer–bacteria assembly. The bacteria were then removed, leaving behind surface imprints on the beads (Fig. 18).

Fig. 18Schematic overview of the process of bacteria assembling with the monomer N-acrylchitosan, followed by Pickering emulsion polymerization to form stable bacteria beads which can be used for rebinding. Reprinted with permission from [128], © John Wiley & Sons

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